Specimen Group Guidelines

Low (Control) — Baseline Group

Positions 1, 2, 3 — Low specimens before any High exposure

These three measurements establish the baseline for the low-concentration pool before any exposure to high-concentration specimens. They represent the expected low result free of carryover contamination and form the reference against which test specimens are compared.

High Specimens

Positions 4, 5, 7, 8, 13, 14, 16, 17, 19, 20 — High concentration specimens

High-concentration specimens measured in the sequence. These are not evaluated for carryover themselves but create the contamination exposure that the subsequent Low (Test) specimens are designed to detect.

Low (Test) — Test Group

Positions 6, 9, 10, 11, 12, 15, 18, 21 — Low specimens following High exposure

Low-concentration specimens measured immediately after one or more high-concentration specimens. If carryover is present, these values will be systematically elevated above the baseline. The carryover estimate is derived from comparing this group's mean to the baseline mean.