How It Works

Factor sensitivity is determined by testing serial dilutions of reference plasma (which contains approximately 100% factor activity) mixed with factor-deficient plasma (which contains 0% activity for the specific factor being studied). Each dilution represents a known proportion of reference plasma — for example, a 35% dilution contains 35% reference plasma and 65% factor-deficient plasma, resulting in approximately 35% factor activity.

Dilutions are expressed as percentages of reference plasma. The default study uses 8 dilution levels (25%, 30%, 35%, 40%, 45%, 50%, 60%, and 70%); the module supports custom dilution values and a configurable range of 3 to 15 dilutions. Each dilution is measured in up to 4 replicates, and the mean clotting time per dilution is used for regression analysis.